flag peptide molecular weight 1013.0 g/mol - fluorogenic-peptide-substrate 1012.9 dalton Unveiling the Molecular Weight of the FLAG Peptide: A Comprehensive Guide

peptides-for-meniscus-injury The FLAG peptide, a widely utilized epitope tag in molecular biology, plays a crucial role in protein detection, purification, and analysis. Understanding its molecular weight is fundamental for researchers to accurately interpret experimental results and optimize protocolsFlag peptide. This article delves into the precise molecular weight of the FLAG peptide, exploring its chemical composition, variations, and the significance of this parameter in scientific applicationsPeptide Calculator - Bachem.

The most commonly encountered FLAG peptide, often referred to as the FLAG tag, possesses a specific amino acid sequence: H-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH. This sequence translates to a distinct chemical formula and, consequently, a defined molecular weight. Numerous scientific databases and product specifications consistently report the molecular weight of this standard FLAG peptide to be approximately 1012.97 Daltons (Da).Source, Synthetic ; Molecular Weight,1012.99; Protein Sequence, H-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH ; Purity, > 95% ; Storage, Shipped at 4°C. Store at -20°C for ... This value is derived from its empirical formula, C41H60N10O20, calculated using the atomic masses of its constituent elements.

While 1012.97 is the most prevalent figure, slight variations can be observed across different sources. For instance, some may list it as 1013.0 g/mol or 1012.99, or even round it to approximately 1 kDa3X FLAG tag peptide. dykddddk peptide. These minor discrepancies often arise from differing calculation methods or the precision of the atomic mass data used. Regardless of the precise decimal, the consensus firmly places the molecular weight of the standard FLAG peptide in the low kilodalton rangeFLAG peptide, DYKDDDDK.

Beyond the single FLAG tag, researchers also employ tandem or multiple FLAG tags for enhanced detection or purification efficiency. The 3x FLAG peptide, for example, is a common variant. This longer sequence has a significantly higher molecular weight.FLAG peptide (5 mg) Specifications for the 3x FLAG peptide typically report a molecular weight of approximately 2861.87 Da or 2,861Molecular Formula. C₄₁H₆₀N₁₀O₂₀. Molecular Weight.1012.97. Appearance. White powder. Purity. > 98%. Solubility. Soluble in water or 1% acetic acid. Components ....87 Da, with a corresponding empirical formula of C120H169N31O49SCorresponding author: Mara C Duncan (ORCID. Another variation, the 3x FLAG tag peptide, lists a molecular weight of 2975.84DYKDDDDK Peptide ; Molecular Formula, C41H60N10O ; Molecular Weight,1012.97; Purity, > 95% ; Solubility, Soluble in Ultrapure water under 1mg/ml ; Form .... It's important to note that the exact molecular weight can vary slightly even for these multi-tag versions due to potential differences in linker sequences or terminal modifications.

The accurate determination of the molecular weight is crucial for several reasons within the laboratory setting. For instance, when performing techniques like SDS-PAGE, understanding the molecular weight of a tagged protein helps in predicting its migration pattern and confirming successful tagging. In peptide purification, knowledge of the molecular weight is essential for selecting appropriate chromatography resins and elution strategies. Furthermore, when using FLAG peptides as competitive elution agents in affinity chromatography, their known molecular weight ensures effective displacement of the tagged protein from the resin.

The molecular formula of the FLAG peptide, C41H60N10O20, provides a detailed breakdown of the atoms present, underscoring its complex organic nature. This molecular formula is a critical piece of information for chemists and biochemists involved in synthesis or advanced structural analysis.

In summary, the FLAG peptide is a cornerstone tool in modern molecular biology, and its molecular weight is a key characteristic.Chemical Information, Storage & Stability. Molecular Weight,1012.97, Formula. C41H60N10O20. The standard FLAG peptide (DYKDDDDK) consistently registers a molecular weight around 1012That brings the total size of the tag to1012.9 daltonor roughly 1 kDa (1). The Flag-tag can be added either to the N-terminus or the C-terminus of a protein ( ....97 Da. Variations like the 3x FLAG peptide have considerably higher molecular weights, typically in the range of 2861.Recombinant TET3 (824-1795) protein87 Da.3X FLAG tag peptide. dykddddk peptide This precise molecular weight information, alongside its molecular formula, is indispensable for accurate experimental design and interpretation, ensuring the reliable application of this vital epitope tag in scientific research.