vivit peptide fluorescent VIVIT peptide - phobius-signal-peptide VIVIT inhibited the production of NO and the secretion of IL-12 p70, IL-23, and TNF The VIVIT Peptide: A Potent Inhibitor of NFAT Signaling

vita-peptide-c-quence-serum-2 The VIVIT peptide has emerged as a crucial tool in the scientific community for its ability to selectively inhibit the nuclear factor of activated T cells (NFAT) signaling pathway. This peptide acts as a high-affinity calcineurin binding peptide, effectively blocking the interaction between calcineurin (CN) and NFAT. This targeted inhibition has significant implications across various biological processes, particularly in the realms of immunology and cardiovascular research.

Understanding the Mechanism of Action

At its core, the VIVIT peptide functions by interfering with a critical step in NFAT activationNFAT Inhibitor. Following cellular stimulation, calcineurin, a phosphatase, dephosphorylates NFAT proteins. This dephosphorylation allows NFAT to translocate into the nucleus, where it regulates the expression of numerous genes involved in immune responses, inflammation, and cell differentiation. The VIVIT peptide specifically targets the PxIxIT motif, a conserved sequence within NFAT that is essential for its binding to calcineurin.作者：A Ellert-Miklaszewska·2021·被引用次数：8—We used aVIVIT peptidefor interference in calcineurin binding to NFAT via a conserved PxIxIT motif. VIVIT was expressed as a fusion protein with a green ... By binding to this site, the VIVIT peptide prevents the calcineurin-NFAT interaction without affecting the phosphatase activity of calcineurin itself or its interactions with other substratesVIVIT. Alt name. NFAT regulatory domain. Insert Size (bp). 51; Tag ...Affinity-driven peptide selection of an NFAT inhibitormore selective than cyclosporin A.. This specificity is a key advantage, distinguishing it from broader immunosuppressants like FK506 or Cyclosporin A (CsA).

Key Properties and Variations of the VIVIT Peptide

The VIVIT peptide is often described as a cell-permeable nuclear factor of activated T cells (NFAT) inhibitor作者：H Noguchi·2017·被引用次数：22—This peptide (11R-VIVIT)selectively interferes with calcineurin-NFAT interactionwithout affecting the activity of calcineurin phosphatase and provides .... This characteristic allows it to be readily introduced into cells for experimental purposes.A simplified model of the mechanism of protection induced ... Several variations and derivatives of the VIVIT peptide have been developed to enhance its utility and explore its therapeutic potential:

* 11R-VIVIT: This is a widely studied, cell-permeable NFAT inhibitor. Research has demonstrated its ability to selectively interfere with the calcineurin-NFAT interaction.Therapeutic potential of VIVIT, a selective peptide inhibitor ... Studies have explored its role in conditions like podocyte research and diabetic complications. Furthermore, 11R-VIVIT has been shown to stimulate osteoblastogenesis through the inhibition of the Cn/NFAT signaling pathway in rat calvaria cells. This cell-permeable peptide 11R-VIVIT binds to CaN and specifically blocks its ability to activate NFAT, interrupting NFAT activation and translocation.

* GFP-VIVIT: This variation involves the VIVIT sequence fused with Green Fluorescent Protein (GFP), allowing for visualization and tracking within cells.Structure-Based Optimization of a Peptidyl Inhibitor against ... VIVIT was expressed as a fusion protein with a green fluorescent protein for interference with calcineurin binding to NFAT.

* dNP2-VIVIT: This synthetic peptide has been investigated for its ability to ameliorate experimental autoimmune encephalomyelitis (EAE), a model for multiple sclerosis. dNP2-VIVIT Ameliorates Experimental Autoimmune Encephalomyelitis by inhibiting NFAT function in autoimmune processes.

* Sim-2-VIVIT: This is another cell permeable form of the NFAT inhibitory peptide VIVIT, which has shown inhibitory effects on human CD4 or CD8 T-cell activationNFAT Inhibitor-1 (VIVIT peptide).

Therapeutic Potential and Applications

The selective inhibition of NFAT by the VIVIT peptide has opened avenues for exploring its therapeutic potential in various disease states.

* Cardiovascular Disorders: The therapeutic potential of VIVIT peptide as a selective NFAT inhibitor has been highlighted in the context of cardiovascular disorders. Research suggests that VIVIT can be used for the research of cardiovascular diseases, and its application as a therapeutic agent in these conditions is being investigated. It has been demonstrated that VIVIT appears to be a specific and potent inhibitor of NFAT activation and thus of NFAT-mediated proliferation and inflammation.

* Inflammation and Autoimmunity: The VIVIT peptide plays a role in modulating inflammatory responses作者：H Yu·2007·被引用次数：96—This review describes the development ofVIVIT peptideas a selective NFAT inhibitor and its application as a therapeutic agent in cardiovascular disorders.. Studies have shown that VIVIT inhibited the production of NO and the secretion of IL-12 p70, IL-23, and TNF, suggesting a global role for NFAT in inflammatory gene expression. This makes it a promising candidate for treating autoimmune diseases and chronic inflammatory conditions. NFAT inhibitor is a cell-permeable peptide that effectively blocks calcineurin-mediated dephosphorylation of nuclear factor of activated T cells (NFAT).

* Cancer Research: The delivery of the VIVIT Peptide to Human Glioma Cells has been explored to interfere with calcineurin-NFAT signaling. This points towards potential applications in cancer therapy where NFAT signaling might contribute to tumor growth or progression. Expression of peptide VIVIT in mammalian cells effectively blocks the CN–NFAT interaction and its downstream signaling without directly blocking CN.NFAT Inhibitor | ≥99%(HPLC) | Selleck | その他 阻害剤

* Immunosuppression: The ability of VIVIT to selectively interfere with calcineurin-NFAT interaction without affecting the activity of calcineurin phosphatase offers a precise approach to immunosuppression. This is crucial in preventing transplant rejection and managing autoimmune diseases.

Research and Development

The VIVIT peptide has been instrumental in advancing our understanding of NFAT biology.Delivery of the VIVIT Peptide to Human Glioma Cells to ... - PMC The development of the VIVIT peptide as a selective NFAT inhibitor is a testament to meticulous scientific inquiry. Its use as a specific probe for binding at the targeted site has facilitated the identification of nonpeptide inhibitors. Furthermore, the VIVIT peptide constitutes a highly selective inhibitor of NFAT, enabling direct identification of NFAT target genes in various cell types. The ongoing research into NFAT inhibitor variants and their delivery mechanisms underscores the continued importance of this peptide in fundamental and translational research. The NFAT inhibitor is a high-affinity calcineurin binding peptide that acts as a selective inhibitor of the interaction of calcineurin with the transcription factor.

In summary, the VIVIT peptide and its derivatives represent a significant advancement in the study and manipulation of the NFAT signaling pathway. Its specificity, efficacy, and diverse applications solidify its position as a valuable research tool with considerable therapeutic promise.